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M. SIMION, I. KLEPS, F. CRACIUNOIU, L. SAVU, M. MIU, A. BRAGARU
Silicon Chip Technology – Electrophoretic DNA Analisys
Abstract.
The
development of miniaturised devices for genetic diagnosis is
of great interest for medicine applications. In this paper,
we focus on the design, the experimental technology
and characterization of a new silicon micro-bio-chip
for rapid testing of DNA material. This chip integrates two classical processes
for DNA analysis: polymerase chain reaction – PCR – technique
for fragments amplification and electrophoresis for
separation of DNA fragments respectively. The proposed
device was realized on silicon substrate and it consists of two
reservoirs connected by one channel and two electrodes inside
of reservoirs; the backside electrical circuit allows
the control of temperature variation. The structures were tested using human DNA
and the corresponding analyses time was less than 45
min.
Keywords: biodevices, silicon microfabrication, DNA analysis.
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