M. TRIF, M. MOISEI, A. ROSEANU
Designing of Efficient Lipidic Nanostructures for the Therapy of the Inflammatory Diseases

Abstract.
This study focuses on the possible therapeutic utility of lipid nanostructures as drug carriers in the local treatment of inflammatory disorders. The effect of liposomes on the percutaneous permeation of piroxicam, a nonsteroidal anti-inflammatory drug, across the rat skin was investigated in vitro using modified Franz-type diffusion cells. Different liposomal formulations have been prepared and characterized in order to obtain the highest efficiency of the drug entrapment. Their topical performances have been compared with a non liposomal system containing piroxicam. Our results demonstrated that the incorporation of piroxicam in liposomes modified their ability to permeate through skin. Liposome ability to fuse with Stratum Corneum Lipids Liposomes (hSCLLs) as skin model was tested. Resonance energy transfer (RET) assay between N-(7-nitro-2,1,3-benzodiazol-4-yl) (N-NBD), the energy donor and rhodamine, N-(lissamine Rhodamine B sulfonyl), the energy accept or was developed to monitor the fusion process. The role of the lipid composition on the fusion or lipid mixing properties was investigated in order to obtain suitable formulation for transdermal drug delivery.

Keywords: liposomes, piroxicam, stratum corneum, resonance energy transfer, transdermal drug delivery.

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