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V. SAMPER, Q. RAMADAN
Microfluidic Sample Preparation Fornucleic Acid Analysis
Abstract.
Microfluidic sample preparation of blood and tissue for nucleic acid analysis
involves multiple processing steps. Components are necessary for tissue
dissociation, cell isolation, cell lysis, gene extraction, and target
purification. The sample size, properties, and the level of integration
influence the choice of silicon, glass, or polymer materials in the final
system. In each case fluidic components can operate on a choice of fundamental
principles. An integrated silicon solution providing more than 10 ng of DNA from
1 ml of whole human blood is shown. |